Production of non-natural 5-methylorsellinate-derived meroterpenoids in Aspergillus oryzae

• Jia Tang,
• Yixiang Zhang and
• Yudai Matsuda

Beilstein J. Org. Chem. 2024, 20, 638–644, doi:10.3762/bjoc.20.56

• meroterpenoids, we can access meroterpenoids that have not yet been reported. In this study, we aimed to expand the structural diversity of meroterpenoids through artificial pathway construction. To achieve this, we constructed a series of artificial metabolic pathways by harnessing genes from the funiculolide

Efficient production of clerodane and ent-kaurane diterpenes through truncated artificial pathways in Escherichia coli

• Fang-Ru Li,
• Xiaoxu Lin,
• Qian Yang,
• Ning-Hua Tan and
• Liao-Bin Dong

Beilstein J. Org. Chem. 2022, 18, 881–888, doi:10.3762/bjoc.18.89

• -kaurane skeletons outlined here may provide an attractive route to prepare other privileged diterpene scaffolds. Keywords: artificial pathway; ent-kaurene; Escherichia coli; overproduction; terpentetriene; Introduction Diterpenoids, of which there are over 34,000 members (http://terokit.qmclab.com

• , respectively, in shake-flask fermentation. Results and Discussion Constructing a two-step artificial pathway to overproduce IPP and DMAPP precursors Following the Williams design, phoN and ipk from Shigella flexneri and Thermoplasma acidophilum, respectively, were codon-optimized and synthesized for

• overexpression in E. coli [19]. Isopentenyl diphosphate isomerase (IDI) from E. coli, which balances IPP and DMAPP in vivo, was also included in our construct. To initially test the efficiency of this two-step artificial pathway, we constructed strains DL10001 (phoN, ipk and idi plus the lycopene-producing genes